Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with heme, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 20% (w/v) PEG3350 and 0.1 M Bis-Tris pH 5.5 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D143A | the mutant shows no activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and guaiacol, but about 20% of wild type activity with Reactive Blue 19 and 10% activity with catechol | Escherichia coli |
D143N | the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Escherichia coli |
H215A | the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Escherichia coli |
L246W | the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Escherichia coli |
R232E | the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 40% of wild type activity with guaiacol and 35% activity with catechol | Escherichia coli |
R232L | the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 90% of wild type activity with guaiacol and 50% activity with catechol | Escherichia coli |
S234Y | the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli O157 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating affinity column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli O157 | ? | - |
? | |
catechol + H2O2 + H+ | - |
Escherichia coli | ? | - |
? | |
catechol + H2O2 + H+ | - |
Escherichia coli O157 | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Escherichia coli | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Escherichia coli O157 | ? | - |
? | |
Reactive Blue 19 + H2O2 | - |
Escherichia coli | ? | - |
? | |
Reactive Blue 19 + H2O2 | - |
Escherichia coli O157 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Escherichia coli |
YfeX | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |